Anti-phospho-STAT2 (Tyr689)

Analysis Note

ControlUntreated and IFN alpha/gamma treated HeLa cell lysate.

Application

Research Sub CategoryImmunological Signaling

Anti-phospho-STAT2 (Tyr689) Antibody is an antibody against phospho-STAT2 (Tyr689) for use in Immunoprecipitation.

Research CategoryEpigenetics & Nuclear Function

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho-STAT2 (Tyr689) from IFN alpha treated HeLa cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia. STAT2 (113 kDa), originally purified from the nuclei of a-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta. Knockout mice with a targeted disruption of STAT2 exhibit greater susceptibility to viral infection and altered responses to Type I interferons. Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases.

Immunogen

Epitope: Phosphorylated Tyr689.

KLH-conjugated linear peptide corresponding to mouse STAT2 phosphorylated at Tyr689.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:200 dilution of this antibody detected phospho-STAT2 (Tyr689) in 10 µg of IFN alpha/gamma treated HeLa cell lysate.

Specificity

This antibody recognizes STAT2 phosphorylated at Tyr689.

Other homologies: Rat (89% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~113 kDa observed. An uncharacterized band may appear at ~260 kDa in some lysates.